chinese hamster ovary

1 زیست شناسی:: تخمدان همستر چینی

The basic vector contains features for propagation and selection in E. coli and a multiple cloning site for insertion of interchangeable modules that are cho­ sen for a particular yeast host, including a sequence for vector integration into the fungal genome, a suitable origin of replication, a promoter to drive expression of the heterologous gene, and selectable markers to complement a range of nutritional auxotrophies or to confer resistance to antifungal com­ pounds, such as hygromycin B (Table 3.12). Chinese hamster ovary (CHO) cells are most commonly used because they produce proteins with human­like gly­ cans and have been adapted for growth in high­density suspension cul­ tures in serum­free medium, which not only reduces costs but also facilitates purification of the target protein and reduces the risk of contamination with animal­derived material. CHO cells were transfected with plasmids containing a regulatable MDM2 gene and cultured under conditions that mimicked the late stages of cell culture and in nutrient­limited medium. Under low­oxygen conditions, many cells, including CHO cells, secrete the acidic waste product lactate as they strug­ gle to obtain energy from glucose. CHO cells.

واژگان شبکه مترجمین ایران